Anti-AtpH | ATP synthase subunit c (chloroplastic)

• Background:

  F-type ATPase(ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of thec subunitbuild up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Chlamydomonas reinhardtii

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated peptides derived from AtpH subunit c ofArabidopsis thalianaUniProt:P56760, TAIR:AtCg00140andChlamydomonas reinhardtiiUniProt:Q37304

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Dilution:

  1 : 10 000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile water

• Molecular Weight:

  8 kDa (forArabidopsis thaliana)

• Precautions:

  Please note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur

• References & Citations:

  Schulzet al. (2017). Molecular architecture of the N-type ATPase rotor ring from Burkholderia pseudomallei. EMBO Rep. 2017 Apr;18(4):526-535. doi: 10.15252/embr.201643374.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4