Anti-AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)

• Background:

  ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.

• HS Code:

  38221900

• Gene ID:

  845169

• UniProt:

  P05022

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Begonia sp., Capsicum annuum, Heliobacterium modesticaldum, Oryza sativa, Zea mays

• Not reactive in:

  Nicotiana benthamiana

• Immunogen:

  Recombinant maize chloroplast AtpA P05022

• Clonality:

  Polyclonal

• Applications:

  Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)

• Field of Research:

  ATP synthase

• Dilution:

  1: 200 (IG), 1 : 5 000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  55 kDa

• Additionnal Information:

  Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757

• References & Citations:

  Uflewski et al. (2024) . The thylakoid proton antiporter KEA3 regulates photosynthesis in response to the chloroplast energy status. Nat Commun. 2024 Mar 30;15 (1) :2792. doi: 10.1038/s41467-024-47151-5. Pao et al. (2018) . Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold) Zhang et al. (2017) . Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40 (9) :1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017) . Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95 (4-5) :463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016) . Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015) . Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15 (15) :2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015) . Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928 (15) 00111-4. doi: 10.1016/j.pep.2015.05.006.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity:

  Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us

• CAS Number:

  9007-83-4