Anti-AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)

Anti-AtpA | Alpha subunit of ATP synthase, chloroplastic (plant)

• Background: ATP synthaseis the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.AtpAis the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Begoniasp., Capsicum annuum, Heliobacterium modesticaldum, Oryza sativa, Zea mays
• Not reactive in: Nicotiana benthamiana
• Immunogen: Recombinant maize chloroplast AtpAP05022
• Clonality: Polyclonal
• Applications: Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1: 200 (IG), 1 : 5 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 55 kDa
• References & Citations: Uflewskiet al. (2024). The thylakoid proton antiporter KEA3 regulates photosynthesis in response to the chloroplast energy status. Nat Commun. 2024 Mar 30;15(1):2792. doi: 10.1038/s41467-024-47151-5.Chenet al. (2023) Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice. Int J Mol Sci. 2023 Mar 18;24(6):5796. doi: 10.3390/ijms24065796.Harchouniet al. (2022) Guanosine tetraphosphate (ppGpp) accumulation inhibits chloroplast gene expression and promotes super grana formation in the moss Physcomitrium (Physcomitrella) patens. New Phytol. 2022;236(1):86-98. doi:10.1111/nph.18320.Kuanget al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Paoet al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhanget al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeonet al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murchaet al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejoet al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yanget al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.