Anti-AtpH | ATP synthase subunit c (chloroplastic)

Anti-AtpH | ATP synthase subunit c (chloroplastic)

• Background: F-type ATPase(ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of thec subunitbuild up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified c subunit fromSpinacia oleraceaUniProt:P69447.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000-1 : 10 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 200 µl of sterile water
• Molecular Weight: 8 kDa (forArabidopsis thaliana)
• Precautions: Note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur
• References & Citations: Nathet al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.Lawrenceet al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.