Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated (40 µg)

Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated (40 µg)

• Background: VDACproteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Beta vulgaris, Brassica oleraceavar. botrytis, Oryza sativa, Papaversp. pollen tubes (IL),Spinacia oleracea,Physcomitrium patens
• Not reactive in: Chlamydomonas reinhardtii, Glycine max, Zea mays,diatoms, Saccharomyces cerevisiae
• Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC proteins includingArabidopsis thalianaVDAC1 UniProt:Q9SRH5, TAIR:AT3G01280, VDAC2  UniProtF4K3R8-1, TAIR:AT5G67500, VDAC3 UniProt:Q9SMX3-1, TAIR:AT5G15090,     VDAC4 UniProt:Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt:Q9M2W6-1, TAIR:AT3G49920
• Clonality: Polyclonal
• Applications: 2D Blue Native PAGE (2D BN-PAGE), Immunolocalization (IL), Western blot (WB)
• Dilution: 1 : 500 (IL), 1 : 2000, 2-30 µg protein/lane (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
• Format: Liquid
• Molecular Weight: 29 kDa (forArabidopsis thaliana)
• Precautions: Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are availablehereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
• Storage Conditions: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.