Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5

Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5

• Background: VDACproteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Amaranthus palmeri, Beta vulgaris, Brassica oleraceavar. botrytis, Brassica rapasubsp.rapa, Citrus sinensis, Fortunella margaritaSwingle,Oryza sativa, Papaversp. pollen tubes (IL),Spinacia oleracea,Physcomitrium patens, Zea mays
• Not reactive in: Chlamydomonas reinhardtii, Glycine max,diatoms, Saccharomyces cerevisiae
• Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC proteins includingArabidopsis thalianaVDAC1 UniProt:Q9SRH5, TAIR:AT3G01280, VDAC2  UniProtF4K3R8-1, TAIR:AT5G67500, VDAC3 UniProt:Q9SMX3-1, TAIR:AT5G15090,     VDAC4 UniProt:Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt:Q9M2W6-1, TAIR:AT3G49920
• Clonality: Polyclonal
• Applications: 2D Blue Native PAGE (2D BN-PAGE), Western blot (WB)
• Dilution: 1 : 500 (IL), 1 : 5000, 2-30 µg protein/lane (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 29 kDa (forArabidopsis thaliana)
• Precautions: Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are availablehereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
• References & Citations: Soriaet al. (2024).Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants. Current Plant Biology Volume 37, March 2024, 100322.Wittmannat al. (2024). Dual plastid targeting of Protoporphyrinogen Oxidase 2 in Amaranthaceae promotes herbicide tolerance. Plant Physiol. 2024 Feb 8:kiae062.doi: 10.1093/plphys/kiae062.Baoet al. (2023). Aberrant accumulation of ceramides in mitochondria trigger cell death requiring autophagy in Arabidopsis. J Exp Bot . 2023 Dec 9:erad456.doi: 10.1093/jxb/erad456.Xueet al.(2023). The PtdIns3P phosphatase MtMP promotes symbiotic nitrogen fixation via mitophagy in Medicago truncatula. iScience. 2023 Sep 15;26(10):107752.doi: 10.1016/j.isci.2023.107752.Belykhet al. (2022) Responses of genes of DNA repair, alternative oxidase, and pro-/antioxidant state in Arabidopsis thaliana with altered expression of AOX1a to gamma irradiation. Int J Radiat Biol. 2022;98(1):60-68. doi: 10.1080/09553002.2022.1998712. Epub 2021 Nov 11. PMID: 34714725.Liet al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.Tarasenkoet al. (2020). Plant mitochondrial subfractions have different ability to import DNA. Theor. Exp. Plant Physiol. doi.org/10.1007/s40626-020-00167-wGarmashet al. (2020). Altered levels of AOX1a expression result in changes in metabolic pathways in Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation. Plant Sci. 2020 Feb;291:110332. doi: 10.1016/j.plantsci.2019.110332.Baiet al. (2019). Overexpression of soybean GmPLD? enhances seed oil content and modulates fatty acid composition in transgenic Arabidopsis. Plant Science Volume 290, January 2020, 110298.Klingeret al. (2019). The signal distinguishing between targeting of outer membrane Beta-barrel protein to plastids and mitochondria in plants. Biochim Biophys Acta Mol Cell Res. 2019 Jan 8;1866(4):663-672. doi: 10.1016/j.bbamcr.2019.01.004.Zhuet al. (2018). A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function. Hortic Res. 2018 Feb 7;5:6. doi: 10.1038/s41438-017-0014-x.Kanget al. (2018). Autophagy-related (ATG) 11, ATG9 and the phosphatidylinositol 3-kinase control ATG2-mediated formation of autophagosomes in Arabidopsis. Plant Cell Rep. 2018 Jan 19. doi: 10.1007/s00299-018-2258-9.Wangand Auwerx (2017). Systems Phytohormone Responses to Mitochondrial Proteotoxic Stress. Mol Cell. 2017 Nov 2;68(3):540-551.e5. doi: 10.1016/j.molcel.2017.10.006.Yinet al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.de Micheleet al. (2016). Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016 Mar 4;15(3):900-13. doi: 10.1021/acs.jproteome.5b00876. Epub 2016 Feb 4.Liet al. (2015). A Chaperone Function of NO CATALASE ACTIVITY1 Is Required to Maintain Catalase Activity and for Multiple Stress Responses in Arabidopsis. Plant Cell. 2015 Feb 19. pii: tpc.114.135095.Rureket al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Armbrusteret al. (2014). Ion antiport accelerates photosynthetic acclimation in fluctuating light environments. Nat Commun. 2014 Nov 13;5:5439. doi: 10.1038/ncomms6439Hsuehet al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725.Takahashiet al. (2014). Transport of rice cyclobutane pyrimidine dimer (CPD) photolyase into mitochondria relies on a targeting sequence located in its C-terminal internal region.Alcantar-Aguirreet al.(2013).ATP produced by oxidative phosphorylation is channeled toward hexokinase bound to mitochondrial porin (VDAC) in beetroots (Beta vulgaris). Planta, March 17.Langet al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.