Anti-VDAC1-5 | Voltage-dependent anion-selective channel pro

• Background:

  VDACproteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Beta vulgaris, Brassica oleraceavar. botrytis, Oryza sativa, Papaversp. pollen tubes (IL),Spinacia oleracea,Physcomitrium patens

• Not reactive in:

  Chlamydomonas reinhardtii, Glycine max, Zea mays,diatoms, Saccharomyces cerevisiae

• Immunogen:

  KLH-conjugated peptide conserved in all known higher plant VDAC proteins includingArabidopsis thalianaVDAC1 UniProt:Q9SRH5, TAIR:AT3G01280, VDAC2  UniProtF4K3R8-1, TAIR:AT5G67500, VDAC3 UniProt:Q9SMX3-1, TAIR:AT5G15090,     VDAC4 UniProt:Q9FKM2-1, TAIR:AT5G57490, VDAC5 UniProt:Q9M2W6-1, TAIR:AT3G49920

• Clonality:

  Polyclonal

• Applications:

  2D Blue Native PAGE (2D BN-PAGE), Immunolocalization (IL), Western blot (WB)

• Dilution:

  1 : 500 (IL), 1 : 5000, 2-30 µg protein/lane (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.

• Format:

  Liquid

• Molecular Weight:

  29 kDa (forArabidopsis thaliana)

• Precautions:

  Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are availablehereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

• Storage Conditions:

  Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

• CAS Number:

  9007-83-4