Anti-ASN | Glutamine-dependent asparagine synthetase

• Background:

  ASN (Glutamine-dependent asparagine synthetase)is essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem. ASN2 is expressed in leaf and ASN1 is expressed in floral organs. The amino acid sequences ofArabidopsis thalianaASN1 and ASN2 are 76% identical. The amino acid sequences ofArabidopsis thalianaandZea maysASN2 are 73.6% identical.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Zea mays

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified full length, tag cleaved, recombinantArabidopsis thalianaASN2, UniProt:Q9LV77, TAIR:AT5G65010

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  assay dependent (ELISA), 1: 100-1: 500, paraffin sections (IHC), 1: 1000-1: 2000 (WB)

• Purity:

  Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

• Format:

  Liquid at 2 mg/mL.

• Molecular Weight:

  65 | 65 kDa

• References & Citations:

  Gaufichonet al. (2017). ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds. Plant J. 2017 Aug;91(3):371-393. doi: 10.1111/tpj.13567.Gaufichonet al. (2013). Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth. Plant Cell Environ. 2013 Feb;36(2):328-42. doi: 10.1111/j.1365-3040.2012.02576.x.

• Storage Conditions:

  Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4