Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

• Background: PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC. Synonymes: PSBQ, PSBQA
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Hordeum vulgare, Picea abies, Pisum sativum, Triticum aestivum, Zea mays
• Not reactive in: Chlamydomonas reinhardtii, Physcomitrella patens, Zostera marina
• Immunogen: KLH-conjugated synthetic peptide derived from available PsbQ protein sequences includingArabidopsis thalianaAt4g21280.Peptide used to elicit this antibody is conserved in both isoforms,Arabidopsis thalianaPsbQ1 and PsbQ2.
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 200 µl of sterile water
• Molecular Weight: 23.8 | 16 kDa
• Precautions: This product can be sold with ProClin if requested
• References & Citations: Charraset al. (2024). An efficient protocol for extracting thylakoid membranes and total leaf proteins from Posidonia oceanica and other polyphenol-rich plants. Plant Methods. 2024 Mar 11;20(1):38. doi: 10.1186/s13007-024-01166-7.Trottiet al. (2024). Physiological Responses to Salt Stress at the Seedling Stage in Wild (Oryza rufipogon Griff.) and Cultivated (Oryza sativa L.) Rice Plants (Basel). 2024 Jan 26;13(3):369. .Yang-ErChen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Pavlovičet al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Albaneseet al. (2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.Grasslet al. (2012). Early events in plastid protein degradation in stay-green Arabidopsis reveal differential regulation beyond the retention of LHCII and chlorophyll. J. Proteome Res. October 2.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.