Discontinued PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein)

Discontinued PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein)

• Background: PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana,Chlamydomonas reinhardtii,Chlorella sp.DT, Chlorella vulgaris, Echinochloa crus-galli, Eucalyptus grandis x Eucalyptus camaldulensis,Festuca arundinaceacv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Nicotiana benthamiana, Oryza sativa, Panicum miliaceum, Picea abies, Pinus banksiana, Pisum sativum, Spinacia oleracea,Solanum tuberosumcultivar Taedong Valley,Synechococcussp. PCC7002, sp. PCC 7942 and sp. PCC6803,Thellungiella salsuginea, Triticum aestivum, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Native purified 33 kDa protein fromSpinacia oleraceaUniProt:P12359
• Clonality: Polyclonal
• Applications: Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1 : 2000-1 : 5000 (WB)
• Purity: Total IgG. Protein G purified in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 35 | 33 kDa
• Precautions: This antibody can be used as a loading control forChlamydomonas reinhardtiiwhile it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schultet al. 2007).
• References & Citations: Turcet al. (2024). Non-photochemical quenching upregulation improves water use efficiency and reduces whole plant level water consumption under drought. J Exp Bot. 2024 Mar 12:erae113. doi: 10.1093/jxb/erae113.Nagyet al. (2023). Photoautotrophic and sustained H2 production by the pgr5 mutant of Chlamydomonas reinhardtii in simulated daily light conditions. International Journal of Hydrogen Energy Volume 53, 31 January 2024, Pages 760-769.Cecchinet al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Loudyaet al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Terentyev(2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..Tangel al. (2020). OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020 May 4;53(3):272-286.e7. doi: 10.1016/j.devcel.2020.03.009.Smytherset al. (2019). Characterizing the effect of Poast on Chlorella vulgaris, a non-target organism. Chemosphere Volume 219, March 2019, Pages 704-712.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.