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Complement Component C5a Mouse Recombinant Protein

CAT: 0519-PROTP06684-01Size: 2 µgDry Ice: NoHazardous: No
CAT#:0519-PROTP06684-01Size:2 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
C5a Mouse Recombinant produced in E. coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa. The Mouse C5a is purified by proprietary chromatographic techniques.
Synonyms
Complement C5; Hemolytic complement; C5; Hc; He; C5a
Gene Name
C5
UniProt
P06684
Reactivity
Mouse
Source
Escherichia coli
Sequence
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Purification
Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Bioactivity
The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution
It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Shipping Conditions
Available
Storage Conditions
Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Other Gene Names
Complement C5
Subcellular Location
Secreted.

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