Mouse CD22/Siglec 2 ELISA Kit PicoKine®, Carrier-free

Mouse CD22/Siglec 2 ELISA Kit PicoKine®, Carrier-free

• Background: CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors (BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.
• Description: Mouse CD22/Siglec 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: B-cell receptor CD22;B-lymphocyte cell adhesion molecule;BL-CAM;Sialic acid-binding Ig-like lectin 2;Siglec-2;T-cell surface antigen Leu-14;CD22;Cd22;Lyb-8, Siglec2;
• Gene Name: B-cell receptor CD22
• Gene ID: 12483
• UniProt: P35329
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S22-R702
• Tissue Specificity: B-lymphocytes.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse CD22
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	240	242	251	262	248	8.69	3.5%
  Sample 2	2070	1778	1779	2120	1936	159.23	8.2%
  Sample 3	4058	4096	4754	4460	4342	284.97	6.5%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 96582 MW
• Subcellular Location: Cell membrane; Single-pass type I membrane protein.
• Sequence Similarities: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
• Protein Name: B-cell receptor CD22