Rat MCP-1 ELISA Kit PicoKine®, Carrier-free

Rat MCP-1 ELISA Kit PicoKine®, Carrier-free

• Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3
• Description: Rat MCP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat CCL2 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-C motif chemokine 2;Immediate-early serum-responsive protein JE;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Small-inducible cytokine A2;CCL2;Je, Mcp1, Scya2;
• Gene Name: C-C motif chemokine 2
• Gene ID: 24770
• UniProt: P14844
• Reactivity: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q24-N148
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants and serum.
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Chemotactic factor that attracts monocytes, but not neutrophils.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 16460 MW
• Specificity: Natural and recombinant rat MCP-1
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	41	37	39	42	39	1.92	4.9%
  Sample 2	257	231	216	228	233	14.94	6.4%
  Sample 3	473	462	446	461	460	9.6	2%

  *number of samples for each test n=16.
• Subcellular Location: Secreted.
• Protein Name: C-C motif chemokine 2