Mouse MCP-1 PicoKine® Quick ELISA Kit, Carrier-free

Mouse MCP-1 PicoKine® Quick ELISA Kit, Carrier-free

• Background: Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.
• Description: Mouse MCP-1 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: C-C motif chemokine 2;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Platelet-derived growth factor-inducible protein JE;Small-inducible cytokine A2;Ccl2;Je, Mcp1, Scya2;
• Gene Name: C-C motif chemokine 2
• Gene ID: 20296
• UniProt: P10148
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q24-R96
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants and serum.
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <6 pg/ml
• Function: Chemotactic factor that attracts monocytes, but not neutrophils.
• Components: Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 16326 MW
• Specificity: Natural and recombinant mouse MCP-1
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	39	36	34	34	35	2.04	5.8%
  Sample 2	224	228	228	219	224	3.69	1.6%
  Sample 3	394	375	431	394	398	20.3	5.1%

  *number of samples for each test n=16.
• Subcellular Location: Secreted.
• Protein Name: C-C motif chemokine 2