Human MCP-1 / CCL2 ELISA Kit PicoKine®

• Background:

  Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

• Description:

  Human MCP-1 / CCL2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2; CCL2; MCP1, SCYA2

• Gene Name:

  C-C motif chemokine 2

• UniProt:

  P13500

• Reactivity:

  Human

• Cross Reactivity:

  There is cross-reactivity with human Eotaxin, MCP-3<1%.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: Q24-T99

• Applications:

  ELISA

• Field of Research:

  Angiogenesis, Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Chemokines, Heart Disease, Immunology, Innate Immunity, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Vascular Inflammation

• Sample Type:

  Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

• Detection Range:

  15.6 pg/ml - 1,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  CCL2

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the intercrine beta (chemokine CC) family.

• Protein Name:

  C-C motif chemokine 2