Recombinant Cynomolgus Thymic Stromal Lymphopoietin/TSLP (C-6His)

• Background:

  Thymic stromal lymphopoietin (TSLP) is a protein belonging to the cytokine family, contains 140 amino acids. It is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. TSLP induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c+ dendritic cells. It can induce allergic inflammation by directly activating mast cells. TSLP is produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromal-like cells. These cells are located in regions where TSLP activity is required.

• Abbreviation:

  TSLP

• Synonyms:

  TSLP; Thymic stroma-derived lymphopoietin; Thymic stromal lymphopoietin

• UniProt:

  A0A7N9CAT7

• Accession Number:

  XP_005557555.1

• Expression System:

  E. coli

• Tag:

  C-His

• Sequence:

  Tyr29-Gln159 (Glu37Gln)

• Applications:

  BLI

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Loaded Anti-Human TSLP mAb-Fc on Protein A Biosensor, can bind Cynomolgus TSLP-His with an affinity constant of 0.07 nM as determined in BLI assay.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  16.2 kDa

• Observed Molecular Weight:

  16 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 6% Trehalose, 2% Glycine, 50mM NaCl, 0.05% Tween 80, pH7.5.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Cynomolgus macaques