Recombinant Human/Cynomolgus VEGFA/VEGF165 Protein

• Background:

  Vascular endothelial growth factor (VEGF) ; also known as vascular permeability factor (VPF) and VEGF-A; is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF) /vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects; including mediating increased vascular permeability; inducing angiogenesis; vasculogenesis and endothelial cell growth; promoting cell migration; inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability; thus it was originally referred to as vascular permeability factor.

• Synonyms:

  MVCD1; VEGF; VEGF165; VPF; Vascular Endothelial Growth Factor Isoform 165

• UniProt:

  P15692

• Accession Number:

  P15692-4

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Met 1-Arg191

• Applications:

  Cell culture

• Field of Research:

  Cardiovascular, Signal Transduction, Cancer, Developmental Biology, Kits, Lysates, Other, Metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 4-16 ng/mL.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  19.2 kDa

• Observed Molecular Weight:

  20&22 kDa

• Species:

  Human