Recombinant Cynomolgus FGL1 (C-6His)

• Background:

  Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1), is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. It is secreted by the liver and functions as a mitogen for hepatocytes. Hepassocin may play a role in the development of hepatocellular carcinomas. Hepassocin is a disulfide-linked homodimeric protein with a C-terminal fibrinogen domain. It is reported that it is a major immune inhibitory ligand of LAG-3 .

• Abbreviation:

  FGL1

• Synonyms:

  425O18-1; Angiopoietin-like protein 4; Angiopoietin-related protein 4; Angptl4; Farp; Fasting-induced adipose factor; Fiaf; HFARP; Hepatic fibrinogen/angiopoietin-related protein; Ng27; Secreted protein Bk89

• UniProt:

  A0A2K5X990

• Accession Number:

  A0A2K5X990

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Leu23-Ile312

• Applications:

  BLI

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Loaded Cynomolgus LAG-3-Fc (Cat#PKSQ050015) on Protein A Biosensor, can bind Cynomolgus FGL1-His (Cat#PKSQ050095) with an affinity constant of 28.6nM as determined in BLI assay.

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  34.8 kDa

• Observed Molecular Weight:

  35 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 300mM NaCl, 50% Glycerol, 0.05% Tween 80, pH 8.5.

• Species:

  Cynomolgus macaques