Recombinant Mouse TPBG/5T4 (C-6His)

• Background:

  Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

• Abbreviation:

  TPBG, 5T4

• Synonyms:

  5T4 Protein;5T4AG Protein; Human; M6P1 Protein; WAIF1 Protein

• UniProt:

  Q9Z0L0

• Accession Number:

  Q9Z0L0

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Ser32-Ser361

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  36.6 kDa

• Observed Molecular Weight:

  50 - 70 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of PBS, pH7.4.

• Species:

  Mouse