Unconjugated TPBG / 5T4 Recombinant Protein

• Background:

  Trophoblast glycoprotein (TPBG), also known as 5T4, is the therapeutic target of several anticancer agents currently in clinical development, largely due to its high expression in tumors and low expression in normal adult tissues.This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development.

• NCBI Gene ID:

  7162

• Swiss Prot:

  Q13641

• Accession Number:

  NP_001159864.1

• Tag:

  His Tag & Avi Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  39.0 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  Trophoblast glycoprotein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hu G, et al., 2015, Mol Pharm., 12 (6), 1730-7.

• Background Reference 02:

  Stern PL, et al., 2017, Cancer Immunol Immunother., 66 (4), 415-426.

• Background Reference 03:

  Stern PL, et al., 2014, Semin Cancer Biol., 29, 13-20.

• Other Product Names:

  TPBG,5T4, M6P1,5T4AG, WAIF1,5T4 oncofetal antigen, Trophoblast glycoprotein,5T4 oncofetal trophoblast glycoprotein

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3