Recombinant Human HMGB1 protein(His Tag)

Recombinant Human HMGB1 protein(His Tag)

• Background: High mobility group protein B1 is a member of the HMGB family consisting of three members; HMGB1; HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein; HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile; it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte; T cell; dendritic cell activities in inflammatory responses.
• Abbreviation: HMGB1
• Synonyms: HMG-1, HMG1, HMG3, SBP-1
• CAS Number: 9000-83-3
• UniProt: P09429
• Accession Number: P09429
• Host: E. coli
• Origin Species: Human
• Tag: C-His
• Sequence: Met 1-Glu 215
• Applications: Cell culture
• Field of Research: Epigenetics and Nuclear Signaling
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce TNF alpha in RAW264.7 cells.The ED₅₀ for this effect is <10 μg/mL.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 35 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.