Recombinant Human HMGB1 Protein

• Background:

  High mobility group protein B1 is a member of the HMGB family consisting of three members; HMGB1; HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein; HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile; it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte; T cell; dendritic cell activities in inflammatory responses.

• Abbreviation:

  HMGB1

• Synonyms:

  HMG-1; HMG1; HMGB1; High Mobility Group Protein 1; High Mobility Group Protein B1

• UniProt:

  P09429

• Accession Number:

  P09429

• Expression System:

  E.coli

• Tag:

  N-MARI

• Sequence:

  Pro92-Val176

• Field of Research:

  Epigenetics and nuclear signal

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  10.0 kDa

• Observed Molecular Weight:

  13 kDa

• Species:

  Human