Recombinant HMGB1 Monoclonal Antibody

• Background:

  High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFB family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.

• Abbreviation:

  HMGB1

• UniProt:

  P09429

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  A synthetic peptide of human HMGB1

• Target:

  Amphoterin; Chromosomal protein; nonhistone; HMG1; DKFZp686A04236; High mobility group 1; High mobility group box 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG-1; HMG1; HMG3; HMGB 1; HMGB1; HMGB1; NONHISTONE CHROMOSOMAL PROTEIN HMG1; SBP 1; Sulfoglucuronyl carbohydrate binding protein

• Clonality:

  Monoclonal

• Clone:

  R07-2D8

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC-P

• Field of Research:

  Cancer; Epigenetics and Nuclear Signaling

• Purification:

  Affinity Purified

• Concentration:

  300 μg/mL

• Dilution:

  WB 1:1000-1:2000; IHC 1:50-1:100

• Buffer:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  25kDa

• Observed Molecular Weight:

  25kDa

• Isotype:

  IgG