Recombinant Human HLA-A*0201 HPV16 E7 complex Protein (C-10His)

• Background:

  The HPV16 E7 protein plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. E7 protein has both transforming and trans-activating activities. It plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha.

• Abbreviation:

  HLA-A*0201 HPV16 E7 complex

• Synonyms:

  HLA-A*0201 HPV16 E7 complex

• UniProt:

  P61769; P01892

• Accession Number:

  P61769&P01892

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  YMLDLQPET&Ile21-Met119&Gly25-Ile308 (Ala269Val)

• Field of Research:

  Microbiology; immunology; Cardiovascular; Cancer

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  49.1 kDa

• Observed Molecular Weight:

  55 - 60 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 500mM NaCl, 0.06% Tween 80, pH7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human