Recombinant Human HLA-A*0201 WT-1 complex Protein (C-10His)

• Background:

  The WT1 protein plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

• Abbreviation:

  HLA-A*0201 WT-1 complex

• Synonyms:

  HLA-A*0201 WT-1 complex

• UniProt:

  P61769; P01892

• Accession Number:

  P61769&P01892

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  RMFPNAPYL&Ile21-Met119&Gly25-Ile308 (Ala269Val)

• Field of Research:

  Microbiology; immunology; Cardiovascular; Cancer

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  49.1 kDa

• Observed Molecular Weight:

  55 - 60 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween 80, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human