Recombinant Human CTCF Protein

• Background:

  Transcriptional Repressor CTCF (CTCF) belongs to the CTCF Zinc-Finger Protein family. CTCF contains twelve C2H2-type zinc fingers and interacts with CHD8. CTCF is widely expressed in many tissues, and it is absent in primary spermatocytes. CTCF is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. CTCF plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. In addition, CTCF is also indispensable in the epigenetic regulation and chromatin remodeling.

• Abbreviation:

  CTCF

• Synonyms:

  11-Zinc Finger Protein; CCCTC-Binding Factor; CTCF; CTCFL Paralog; Transcriptional Repressor CTCF

• UniProt:

  P49711

• Accession Number:

  P49711

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Met 1-Ile154

• Field of Research:

  Epigenetics and nuclear signal

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  16.9 kDa

• Observed Molecular Weight:

  35-40 kDa

• Species:

  Human