Recombinant Human Insulin Protein

• Background:

  After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake.

• Synonyms:

  IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10

• UniProt:

  P01308

• Accession Number:

  P01308

• Expression System:

  Yeast

• Tag:

  None

• Sequence:

  Phe25-Lys53&Gly90-Asn110

• Applications:

  Cell culture

• Field of Research:

  Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 0.2-1 µg/mL.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  7.1 kDa

• Observed Molecular Weight:

  6 kDa

• Species:

  Human