Recombinant Cytochrome C Monoclonal Antibody

• Background:

  Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

• Abbreviation:

  Cytochrome C

• UniProt:

  P99999

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  A synthetic peptide of human Cytochrome C

• Target:

  Cytochrome C; Somatic; CYC; Cytochrome C; THC4; HCS

• Clonality:

  Monoclonal

• Clone:

  R09-6H3

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC-P

• Field of Research:

  Cancer; Cell Biology; Cardiovascular; Metabolism; Signal Transduction

• Purification:

  Affinity Purified

• Concentration:

  300 μg/mL

• Dilution:

  WB 1:1000-1:2000; IHC 1:50-1:100

• Buffer:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  12kDa

• Observed Molecular Weight:

  14kDa

• Citation 01:

  https://onlinelibrary.wiley.com/doi/abs/10.1111/and.14185

• Isotype:

  IgG