Recombinant Human Cytochrome b5/CYB5A Protein (His Tag)

• Background:

  Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.

• Abbreviation:

  Cytochrome b5; CYB5A

• Synonyms:

  CYB5; CYB5A; Cytochrome b5; MCB5; Microsomal Cytochrome b5 Type A

• UniProt:

  P00167

• Accession Number:

  P00167

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Met 1-Asp134

• Field of Research:

  Signal Transduction; Cancer; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.5 kDa

• Observed Molecular Weight:

  17 kDa

• Species:

  Human