Recombinant Human Cytochrome C/CYCS Protein (His Tag)

• Background:

  Cytochrome C (CYCS) is a small heme protein that belongs to the cytochrome c family. It is found loosely associated with the inner membrane of the mitochondrion. Cytochrome C is a highly soluble protein that functions as a central component of the electron transport chain in mitochondria. CYCS transfers electrons between Complexes III (Coenzyme Q - Cyt C reductase) and IV (Cyt C oxidase) . CYCS plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of Cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

• Abbreviation:

  Cytochrome C; CYCS

• Synonyms:

  CYC; CYCS; Cytochrome C

• UniProt:

  P99999

• Accession Number:

  P99999

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Gly2-Glu105

• Field of Research:

  Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  12.8 kDa

• Observed Molecular Weight:

  16 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 200mM NaCl, 50% Glycerol, 0.05% Tween 80, pH7.0.

• Species:

  Human