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Vimentin Polyclonal Antibody

CAT: 0763-E-AB-70081-01Size: 60 µLDry Ice: NoHazardous: No
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CAT#:0763-E-AB-70081-01Size:60 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Abbreviation
Vimentin
UniProt
P08670, P20152, P31000
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse vimentin
Target
CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; Vimentin
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Cancer; Developmental Biology; Neuroscience; Signal Transduction; Stem Cells
Purification
Affinity purification
Concentration
1.1 mg/mL
Dilution
WB 1:500-1:2000; IHC 1:200-1:800
Buffer
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
57kDa
Observed Molecular Weight
57kDa
Citation 01
https://www.mdpi.com/2055064
Citation 02
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068511/
Citation 03
https://www.hindawi.com/journals/cjgh/2022/5332129/
Isotype
IgG

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