Vimentin Polyclonal Antibody

• Background :

  Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

• Abbreviation :

  Vimentin

• UniProt :

  P08670, P20152, P31000

• Host :

  Rabbit

• Reactivity :

  Human; Mouse; Rat

• Immunogen :

  KLH conjugated Synthetic peptide corresponding to Mouse vimentin

• Target :

  CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; Vimentin

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Applications :

  WB; IHC

• Field of Research :

  Cancer; Developmental Biology; Neuroscience; Signal Transduction; Stem Cells

• Purification :

  Affinity purification

• Concentration :

  1.1 mg/mL

• Dilution :

  WB 1:500-1:2000; IHC 1:200-1:800

• Buffer :

  PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

• Shipping Conditions :

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions :

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight :

  57kDa

• Observed Molecular Weight :

  57kDa

• Citation 01 :

  https://www.mdpi.com/2055064

• Citation 02 :

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068511/

• Citation 03 :

  https://www.hindawi.com/journals/cjgh/2022/5332129/

• Isotype :

  IgG