Recombinant Vimentin Monoclonal Antibody

• Background:

  Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.

• Abbreviation:

  VIM

• UniProt:

  P08670

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant Human Vimentin Protein

• Target:

  HEL; Epididymis luminal protein; CTRCT; CTRCT30; HEL113; Vimentin; VIM; Epididymis luminal protein 113; VIME; FLJ36605; epididymis secretory sperm binding protein; CTRCT30; HEL113; vimentin; CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; Vimentin

• Clonality:

  Monoclonal

• Clone:

  11G8

• Conjugation:

  Unconjugated

• Applications:

  ICC/IF

• Field of Research:

  Neuroscience; Signal Transduction; Stem Cells; Cancer; Developmental Biology

• Purification:

  Protein A

• Concentration:

  1 mg/mL

• Dilution:

  ICC/IF 1:20-1:100

• Buffer:

  0.2 μm filtered solution in PBS

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

• Isotype:

  IgG