Recombinant Vimentin Monoclonal Antibody

• Background:

  Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

• Abbreviation:

  Vimentin

• UniProt:

  P08670

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat; Hamster

• Immunogen:

  A synthetic peptide of human Vimentin

• Target:

  CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; Vimentin

• Clonality:

  Monoclonal

• Clone:

  R02-8C4

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC-P; IF

• Field of Research:

  Cancer; Developmental Biology; Neuroscience; Signal Transduction; Stem Cells

• Purification:

  Affinity Purified

• Concentration:

  300 μg/mL

• Dilution:

  WB 1:500-1:1000; IHC 1:50-1:100; IF 1:50-1:100

• Buffer:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  54kDa

• Observed Molecular Weight:

  54kDa

• Isotype:

  IgG