RAF1 Polyclonal Antibody

• Background:

  This gene is the cellular homolog of viral raf gene (v-raf) . The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

• Abbreviation:

  RAF1

• UniProt:

  P04049

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Recombinant fusion protein of human RAF1 (NP_002871.1) .

• Target:

  CMD1NN; CRAF; NS5; Raf-1; c-Raf; RAF1

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; IF

• Field of Research:

  Cancer; Cell Biology; Metabolism; Signal Transduction

• Purification:

  Affinity purification

• Concentration:

  1mg/mL

• Dilution:

  WB 1:500-1:2000; IHC 1:50-1:200; IF 1:50-1:200

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  73kDa/75kDa

• Observed Molecular Weight:

  73kDa

• Citation 01:

  https://febs.onlinelibrary.wiley.com/doi/abs/10.1111/febs.16359

• Isotype:

  IgG