Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

CAT:

763-E-BC-F032-01

Size:

96 Tests

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Field of Research: Glycolysis and Lipid Metabolism
Detection Method: Fluorometric method
Assay Principle: Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Assay Performance Time: 20 min
Detection Range: 0.12-30 μmol/L
Sensitivity: 0.12 μmol/L
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.

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E-BC-F032-01	Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit
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