Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

• Target:

  TC

• Type:

  Colorimetric method

• Field of Research:

  Metabolic Diseases; Glycolysis and Lipid Metabolism

• Assay Principle:

  Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.

• Assay Performance Time:

  50 min

• Sample Type:

  Serum; plasma; hydrothorax; animal tissue

• Detection Range:

  0.09-25.85 mmol/L

• Sensitivity:

  0.09 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332

• Citation 02:

  https://journals.asm.org/doi/abs/10.1128/mBio.01155-21

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S1043661819308473

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S0223523421002373

• Citation 05:

  https://europepmc.org/article/pmc/pmc8689138?javascript_support=no

• Citation 06:

  https://link.springer.com/article/10.1007/s10565-022-09768-4

• Citation 07:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279576/

• Citation 08:

  https://pubs.rsc.org/en/content/articlelanding/2023/fo/d2fo03509j

• Citation 09:

  https://www.sciencedirect.com/science/article/pii/S2212429222005399

• Citation 10:

  https://www.sciencedirect.com/science/article/pii/S0378874122009321

• Citation 11:

  https://www.mdpi.com/1861292

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/S037842742100182X

• Inter CV:

  2.8%

• Intra CV:

  1.1%

• Detection Instrument:

  Spectrophotometer