Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit

• Target:

  T-SH

• Type:

  Colorimetric method

• Field of Research:

  Oxidative stress

• Assay Principle:

  Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.

• Assay Performance Time:

  20 min

• Sample Type:

  Serum; plasma; animal tissue

• Detection Range:

  9.91-1000 μmol/L

• Sensitivity:

  9.91 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S1319562X22004119

• Inter CV:

  2.9%

• Intra CV:

  2.5%

• Detection Instrument:

  Microplate reader