Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit
CAT:
26-K603-100
Size:
100 Assays
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit
- Description: The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both by colorimetric or fluorometric methods. Majority of the cholesterol in blood is in the form of cholesteryl esters which can be hydrolyzed to cholesterol by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase to yield H₂O₂which reacts with a sensitive cholesterol probe to produce color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase in the reaction. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total (cholesterol plus cholesteryl esters).
- Features & Benefits: • Simple procedure; takes ~60 minutes • Fast and convenient
- Shipping Conditions: gel pack
- Storage Conditions: -20°C
- Shelf Life: 12 months