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Human IFNα (Interferon Alpha) CLIA Kit

CAT: 0763-E-CL-H1455-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CL-H1455-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
IFNα
UniProt
P01562
Cross Reactivity
This kit recognizes Human IFNα in samples.No significant cross-reactivity or interference between Human IFNα and analogues was observed
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Immunology; Cancer
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IFNα. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IFNα. You can calculate the concentration of Human IFNα in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human IFNα concentrations in serum, plasma and other biological fluids.

UniProtKB · P0DY56

Interferon alpha-1

IFNA1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P0DY56
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNA1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-alpha-1
EC number
—
Processing
Precursor
Secondary accessions
D4Q9M8, P01562, Q14605, Q2M1L8, Q52LB8, Q5VYQ2, Q7M4Q1, Q8WZ68, Q9UMJ3
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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