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Rat IFN-β (Interferon Beta) CLIA Kit

CAT: 0763-E-CL-R0381-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CL-R0381-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
IFN-β
UniProt
P70499
Cross Reactivity
This kit recognizes Rat IFN-β in samples.No significant cross-reactivity or interference between Rat IFN-β and analogues was observed
Target
IFNB1; IFB; IFF; IFNB
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Cardiovascular; Immunology
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-β. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat IFN-β. You can calculate the concentration of Rat IFN-β in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat IFN-β concentrations in serum, plasma and other biological fluids.

UniProtKB · P70499

Interferon beta

IFNB_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P70499
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ifnb1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
IFN-beta
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinPhosphoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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