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Mouse MT (Melatonin) ELISA Kit

CAT: 0763-E-EL-M0788-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-M0788-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
MT
Cross Reactivity
This kit recognizes Mouse MT in samples.No significant cross-reactivity or interference between Mouse MT and analogues was observed
Target
N-Acetyl-5-Methoxytryptamine
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Metabolism; Neuroscience
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse MT. During the reaction, Mouse MT in the sample or standard competes with a fixed amount of Mouse MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.81-500 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
4.69 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12573
Citation 02
https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12851
Citation 03
https://www.pnas.org/content/115/29/E6880.short
Citation 04
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359097/
Citation 05
https://www.mdpi.com/2073-4409/11/10/1690
Citation 06
https://www.sciencedirect.com/science/article/pii/S0006295222001678
Citation 07
https://www.sciencedirect.com/science/article/pii/S0378517321003896
Citation 08
https://link.springer.com/article/10.1007/s40618-023-02014-6
Citation 09
https://www.mdpi.com/2079-7737/11/7/962
Citation 10
https://www.sciencedirect.com/science/article/pii/S1050464821000644
Citation 11
https://www.nature.com/articles/s41598-020-59568-1
Citation 12
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877689/
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse MT concentrations in serum, plasma and other biological fluids.

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