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Rat MT (Melatonin) ELISA Kit

CAT: 0763-E-EL-R0031-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-EL-R0031-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
MT
Cross Reactivity
This kit recognizes Rat MT in samples.No significant cross-reactivity or interference between Rat MT and analogues was observed
Target
N-Acetyl-5-Methoxytryptamine
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Metabolism; Neuroscience
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MT. During the reaction, Rat MT in the sample or standard competes with a fixed amount of Rat MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
9.38 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12657
Citation 02
https://www.sciencedirect.com/science/article/pii/S1043661823000701
Citation 03
https://europepmc.org/article/med/35402542?javascript_support=no
Citation 04
https://www.hindawi.com/journals/omcl/2022/7420726/
Citation 05
https://www.sciencedirect.com/science/article/pii/S0147651320312380
Citation 06
https://www.mdpi.com/2050596
Citation 07
https://www.mdpi.com/2146196
Citation 08
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247890
Citation 09
https://onlinelibrary.wiley.com/doi/abs/10.1111/bcpt.12652
Citation 10
https://www.tandfonline.com/doi/abs/10.1080/13880209.2021.2019283
Citation 11
https://www.tandfonline.com/doi/abs/10.1080/13880209.2020.1812674
Citation 12
https://www.sciencedirect.com/science/article/pii/S0385814621001322
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat MT concentrations in serum, plasma and other biological fluids.

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