Uncoated Rat MT (Melatonin) ELISA Kit

• Abbreviation:

  MT

• Cross Reactivity:

  This kit recognizes Rat MT in samples.No significant cross-reactivity or interference between Rat MT and analogues was observed

• Target:

  N-Acetyl-5-Methoxytryptamine; MT; Melatonin

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Metabolism; Neuroscience

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MT. During the reaction, Rat MT in the sample or standard competes with a fixed amount of Rat MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  24 h

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  15.63-1000 pg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%

• Recovery:

  80%-120%

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  -20°C,12 months

• Species:

  Rat

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Rat MT concentrations in serum, plasma and other biological fluids.