Products for Research Use Only
RegisterLogin

RecombinantM-CSF, Human

CAT: 0013-GTR18990487-01Size: 1 mgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18990487-01Size:1 mg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis, [1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63.Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Product Name Alternative
Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, M-CSF
Source
Escherichia coli.
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by non-reducing SDS-PAGE.
Bioactivity
ED50 of 1 - 3 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 10ˆ5-1 x 10ˆ6 units/mg.
Form
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.
Molecular Weight
28 kDa, observed by non-reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.
AA Sequence
MEEVSEYCS HMIGSGHLQS LQRLIDSQME TSCQITFEFV DQEQLKDPVC YLKKAFLLVQ DIMEDTMRFR DNTPNAIAIV QLQELSLRLK SCFTKDYEEH DKACVRTFYE TPLQLLEKVK NVFNETKNLL DKDWNIFSKN CNNSFAECSS QGHERQSEGS

Related Products

CatalogName
GTR18990273-01RecombinantM-CSF, Mouse
GTR18990486-01RecombinantM-CSF, Mouse
GTR18990485-01RecombinantM-CSF, Rat
GTR18990272-01RecombinantM-CSF, Rat
GTR18991657-01RecombinantGM-CSF, Human
GTR18992101-01RecombinantG-CSF, Human
GTR17688571G-CSF/CSF3/CSF Rabbit Polyclonal Antibody
GTR17692751G-CSF CSF3/CSF Rabbit Monoclonal Antibody
GTR17689195G-CSF/CSF3/CSF Rabbit Polyclonal Antibody
GTR18880572Recombinant Human GM-CSF

Alternative Products

CatalogName
BK0127RecombinantM-CSF,Human
BK0127-01RecombinantM-CSF, Human
BK0267RecombinantM-CSF,Human(CHO-expressed)
BK0267-01RecombinantM-CSF, Human (CHO-expressed)
Z05639-500M-CSF/CSF-1, Human
PR27079-2M-CSF / CSF-1 Human Protein
MBS691752-01Human GM-CSF
CYT-308-01M CSF Human
RP250001Human G-CSF
HY-P70422-01G-CSF, Human

Popular Products