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RecombinantM-CSF, Rat

CAT: 0013-GTR18990485-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18990485-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. Interaction of M-CSF with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of several types of cancer, including breast and endometrial cancer.Recombinant Rat Macrophage Colony Stimulating Factor (M-CSF) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 155 amino acids each. A fully biologically active molecule, rrM-CSF has a molecular mass of 28 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Product Name Alternative
Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, M-CSF
Source
Escherichia coli.
Field of Research
Immunology & Inflammation
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE.
Bioactivity
ED50 2x10ˆ5 units/mg.
Form
Lyophilized after extensive dialysis against 50mM Tris, 150mM NaCl, pH 8.0.
Molecular Weight
28 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant Rat Macrophage Colony Stimulating Factor (M-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rat M-CSF should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Notes
For research use only.
Applications Notes
Reconstituted in ddH2O at 100 μg/ml.
Preservative
Lyophilized after extensive dialysis against 50mM Tris, 150mM NaCl, pH 8.0.
AA Sequence
MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP

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