Mouse anti Integrin beta 1D

CAT: 0579-MUB0906PSize: 0.1 mgDry Ice: NoHazardous: No

1 / 1

CAT#:0579-MUB0906PSize:0.1 mg

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Background

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated α and β subunits. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migRation and apoptosis. There are two major forms of integrin β1: β1A and β1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. β1A is present in all tissues, except cardiac and skeletal muscle, which instead express the β1D variant.

CAS Number

9007-83-4

UniProt

P05556

Host

Mouse

Species Reactivity

Canine, Human, Mouse, Swine

Isotype

IgG1

Clone

2B1

Type

Primary Antibodies

Source

2B1 is a Mouse monoclonal IgG1, κ antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin β1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.

Applications

ICC, IHC (frozen), WB

Field of Research

Cardiovascular, Cell adhesion

Assay Principle

2B1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

References & Citations

1. van der Flier, A., Gaspar, A. C., Thorsteinsdottir, S., Baudoin, C., Groeneveld, E., Mummery, C. L., and Sonnenberg, A. (1997). Spatial and temporal expression of the beta1D integrin during Mouse development, Dev Dyn 210, 472-86.

Shipping Conditions

Ambient Temperature

Storage Conditions

The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product

UniProtKB · P05556

Integrin beta-1

ITB1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P05556
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ITGB1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A8K6N2, D3DRX9, D3DRY3, D3DRY4, D3DRY5, P78466, P78467, Q13089, Q13090, Q13091, Q13212, Q14622, Q14647, Q29RW2, Q7Z3V1, Q8WUM6

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

CalciumMagnesiumMetal-binding

Biological process

Cell adhesionHost-virus interactionMyogenesis

Cellular component

Cell junctionCell membraneCell projectionEndosomeMembrane

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

Host cell receptor for virus entryIntegrinReceptor

Related Products

Catalog	Name
MUB0909P	Mouse anti Integrin beta 4 (CD104)
MUB0901P	Mouse anti Integrin alpha 3A
MUB0905P	Mouse anti Integrin alpha 6B
MUB0900P	Mouse anti Integrin alpha 3A
MUB0910P	Mouse anti Integrin alpha 5 (CD49e)
MUB2056P	Mouse anti CD11a, Integrin alpha-L
MUB0902P	Mouse anti Integrin alpha 3B (CD49c)
MUB0903P	Mouse anti Integrin alpha 3B + alpha 6B
MUB0110P-CE/IVD	Mouse anti actin beta-cytoplasmic
X1290P	Sheep anti Mouse Bax-beta

Alternative Products

Catalog	Name
MBS570164-01	Mouse anti Integrin beta 1D
MBS570278-01	Mouse anti Integrin beta 4 (CD104)
MBS147010-01	Mouse Anti Human Integrin Beta 1
STJ97287	Anti-Integrin beta antibody
gAP-0052	Rat monoclonal anti-mouse Integrin beta-7
MBS8103275-01	Anti-Integrin beta 1 Antibody, Mouse Monoclonal
mAP-0451	Rat Monoclonal anti-mouse Integrin beta 4
hAP-0423	Mouse Monoclonal anti-human Integrin beta (CD18)
mAP-0452	Rat Monoclonal anti-mouse Integrin beta 7
hAP-0780	Mouse Monoclonal anti-human Integrin beta 6