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Human MUC5B (Mucin 5 Subtype B) Microsample ELISA Kit

CAT: 0013-GTR18873056-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873056-01Size:48 Tests
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Description
Human MUC5B (Mucin 5 Subtype B) Microsample ELISA Kit
Product Name Alternative
MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5, Subtype B, Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin
UniProt
Q9HC84
Reactivity
Human
Field of Research
Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.116 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC5B in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · Q9HC84

Mucin-5B

MUC5B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9HC84
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MUC5B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MUC-5B
EC number
—
Processing
Precursor
Secondary accessions
O00447, O00573, O14985, O15494, O95291, O95451, Q14881, Q7M4S5, Q99552, Q9UE28
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CopperMetal-binding

PTM

Disulfide bondGlycoprotein

Domain

RepeatSignal

Cellular component

Secreted

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