Laemmli Sample Buffer 2X Solution

• Description:

  Laemmli Sample Buffer 2X_x000D_ _x000D_ Catalog number: B2010105_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Volume/Weight: 4 x 2 mL_x000D_ pH: 6.8_x000D_ Supplied as: Ready-to-use solution (mix with sample 1:1)_x000D_ Appearance: Blue Solution_x000D_ Applications: denaturing and loading protein samples during SDS-PAGE._x000D_ Storage: ˆ’20°C_x000D_ Keywords: Laemmli Buffer, SDS-PAGE loading buffer, Protein Denaturing buffer_x000D_ Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Karlsson JO, Ostwald K, KÃ¥björn C, Andersson M. A method for protein assay in_x000D_ Laemmli buffer. Anal Biochem. 1994 May 15;219(1):144-6.2: Sheen H. Quantitation of yeast total proteins in sodium dodecyl sulfate-_x000D_ polyacrylamide gel electrophoresis sample buffer for uniform loading. Anal_x000D_ Biochem. 2016 Apr 1;498:95-7.3: Amponsah A, Nayak B. Evaluation of the efficiency of three extraction_x000D_ conditions for the immunochemical detection of allergenic soy proteins in_x000D_ different food matrices. J Sci Food Agric. 2018 Apr;98(6):2378-2384.4: Cai X, Zheng Y, Speck NA. A Western Blotting Protocol for Small Numbers of_x000D_ Hematopoietic Stem Cells. J Vis Exp. 2018 Aug 22;(138):56855._x000D_ _x000D_ 5: Scully RR, Pressley TA, O€™Neil RG. A site-directed antibody recognizes a_x000D_ component of the ouabain-binding domain of the alpha 1 subunit of rat_x000D_ Na+,K(+)-ATPase. Biochem Cell Biol. 1993 Nov-Dec;71(11-12):538-43._x000D_ _x000D_ 6: Guthrie CR, Murray LW, Kopchok GE, Rosenbaum D, White RA. Biochemical_x000D_ mechanisms of laser vascular tissue fusion. J Invest Surg. 1991;4(1):3-12._x000D_ _x000D_ 7: Rendić D, Wilson IB, Lubec G, Gutternigg M, Altmann F, Léonard R. Adaptation_x000D_ of the €œin-gel release method€ to N-glycome analysis of low-milligram amounts of_x000D_ material. Electrophoresis. 2007 Dec;28(23):4484-92._x000D_ _x000D_ 8: Wang X, Wang H, Wang D, Wang D, Han B, Tian W, Guo A. Thermal denaturation_x000D_ produced degenerative proteins and interfered with MS for proteins dissolved in_x000D_ lysis buffer in proteomic analysis. Electrophoresis. 2011 Feb;32(3-4):348-56._x000D_ _x000D_ Products Related to Laemmli Sample Buffer 2X Solution: Biochemical Buffers and Solutions

• Short Description:

  Catalog Number: B2010105 (4 x 2 mL)_x000D_ Laemmli Sample Buffer 2X is a high quality biotechnology grade research product used for denaturing and loading protein samples during SDS-PAGE. Custom bulk orders of this product are available upon request.

• Weight:

  2

• Length:

  2.5

• Width:

  2

• Height :

  1.2