Terminal Transferase Buffer Solution
| Catalog number: | _x000D_B2010101 | _x000D_
| Lot number: | _x000D_Batch Dependent | _x000D_
| Expiration date: | _x000D_Batch Dependent | _x000D_
| Volume/Weight | _x000D_4 x 1 mL | _x000D_
| pH | _x000D_6.8 | _x000D_
| Supplied as | _x000D_Ready-to-use solution | _x000D_
| Appearance | _x000D_Clear solution | _x000D_
| Applications | _x000D_Optimized buffer for use with Terminal Deoxynucleotidyl Transferase, an enzyme which catalyzes the processive addition of dNTPs to the 3´-OH end of a single-stranded DNA primer. | _x000D_
| Shelf-Life | _x000D_1 year from date of manufacture | _x000D_
| Storage: | _x000D_Keep at 2-8°C | _x000D_
| Keywords: | _x000D_Terminal Transferase Buffer, TT buffer | _x000D_
| Grade | _x000D_Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. | _x000D_
| References | _x000D_1: Liu K, Zhao E, Ilyas G, Lalazar G, Lin Y, Haseeb M, Tanaka KE, Czaja MJ._x000D_ Impaired macrophage autophagy increases the immune response in obese mice by_x000D_ promoting proinflammatory macrophage polarization. Autophagy. 2015;11(2):271-84._x000D_ _x000D_ _x000D_ 2: Tang F, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA._x000D_ RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat_x000D_ Protoc. 2010 Mar;5(3):516-35._x000D_ _x000D_ 3: Gazit N, Vertkin I, Shapira I, Helm M, Slomowitz E, Sheiba M, Mor Y, Rizzoli_x000D_ S, Slutsky I. IGF-1 Receptor Differentially Regulates Spontaneous and Evoked_x000D_ Transmission via Mitochondria at Hippocampal Synapses. Neuron. 2016 Feb_x000D_ 3;89(3):583-97._x000D_ _x000D_ 4: Deng G, Wu R. An improved procedure for utilizing terminal transferase to add_x000D_ homopolymers to the 3' termini of DNA. Nucleic Acids Res. 1981 Aug_x000D_ 25;9(16):4173-88._x000D_ _x000D_ 5: Zhang C, Dai P, Spokoyny AM, Pentelute BL. Enzyme-catalyzed macrocyclization_x000D_ of long unprotected peptides. Org Lett. 2014 Jul 18;16(14):3652-5._x000D_ _x000D_ 6: Yamashita S. Heat-induced antigen retrieval: mechanisms and application to_x000D_ histochemistry. Prog Histochem Cytochem. 2007;41(3):141-200._x000D_ _x000D_ 7: Niyomrattanakit P, Wan KF, Chung KY, Abas SN, Seh CC, Dong H, Lim CC, Chao_x000D_ AT, Lee CB, Nilar S, Lescar J, Shi PY, Beer D, Lim SP. Stabilization of dengue_x000D_ virus polymerase in de novo initiation assay provides advantages for compound_x000D_ screening. Antiviral Res. 2015 Jul;119:36-46._x000D_ _x000D_ 8: Ma LN, Li LD, Li SC, Hao XM, Zhang JY, He P, Li YK. Allicin improves cardiac_x000D_ function by protecting against apoptosis in rat model of myocardial infarction._x000D_ Chin J Integr Med. 2017 Aug;23(8):589-597._x000D_ _x000D_ 9: Zheng J, Liu Z, Li W, Tang J, Zhang D, Tang X. Lithium posttreatment confers_x000D_ neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral_x000D_ hemorrhage rats. J Neurosurg. 2017 Oct;127(4):716-724. | _x000D_
| Related Products | _x000D_Biochemical Buffers and Solutions | _x000D_
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