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Human Endothelin Converting Enzyme 1 (ECE1) ELISA Kit

CAT: 0013-GTR18809512-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18809512-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin Converting Enzyme 1 (ECE1) .
Product Name Alternative
ECE1, ECE-1
UniProt
P42892
Reactivity
Human
Field of Research
Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
11.4 pg/mL
Notes
For research use only.
Applications Notes
Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ECE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ECE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECE1 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
31.25-2000 pg/mL

UniProtKB · P42892

Endothelin-converting enzyme 1

ECE1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P42892
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ECE1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
ECE-1
EC number
3.4.24.71
Processing
—
Secondary accessions
A8K3P1, B4E291, Q14217, Q17RN5, Q2Z2K8, Q58GE7, Q5THM5, Q5THM7, Q5THM8, Q9UJQ6, Q9UPF4, Q9UPM4, Q9Y501
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

Disease

Disease variantHirschsprung disease

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

HydrolaseMetalloproteaseProtease

Ligand

Metal-bindingZinc

Domain

Signal-anchorTransmembraneTransmembrane helix

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