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Mouse Endothelin Converting Enzyme 1 (ECE1) ELISA Kit

CAT: 0013-GTR18857888-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18857888-01Size:48 Tests
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Description
Mouse Endothelin Converting Enzyme 1 (ECE1) ELISA Kit
Product Name Alternative
ECE1
UniProt
Q4PZA2
Reactivity
Mouse
Target
ECE1
Field of Research
Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
9.38 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ECE1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ECE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ECE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ECE1. You can calculate the concentration of Mouse ECE1 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000pg/mL

UniProtKB · Q4PZA2

Endothelin-converting enzyme 1

ECE1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q4PZA2
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ece1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
ECE-1
EC number
3.4.24.71
Processing
—
Secondary accessions
B1AXF9, Q4PZ99, Q4PZA1, Q6P9Q9
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

HydrolaseMetalloproteaseProtease

Ligand

Metal-bindingZinc

Technical term

Reference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix

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